CRONUS-2P is a three-channel tunable femtosecond laser providing watt-level, synchronized outputs with high repetition rates, short pulse durations, and GDD control, making it the ultimate source for nonlinear microscopy. In its standard configuration, the two independently tunable outputs, which can operate separately or simultaneously, cover 680 – 960 nm and 940 – 1300 nm, respectively. The third output, fixed at 1025 nm, is accessible in parallel.

Due to this versatility, CRONUS-2P can be applied for the simultaneous excitation of multiple fluorescent probes, calcium indicators, or opsins at their absorption maxima. Additionally, second- and third‑harmonic emission (SHG and THG) can be spectrally shifted for easier detection or resonant enhancement.

The two tunable and simultaneous channels also enable advanced coherent anti-Stokes and stimulated Raman scattering (CARS and SRS) applications with dual-band imaging, a broader selection of vibrational resonance frequencies, constant-difference dual‑beam tuning, and resonant enhancement.

CRONUS-2P configurations:
Standard: 680 – 960 nm (tunable), 940 – 1300 nm (tunable), and 1025 nm (fixed)
Dual output A: 680 – 960 nm (tunable), 680 – 960 nm (tunable), and 1025 nm (fixed)
Dual output B: 940 – 1300 nm (tunable), 940 – 1300 nm (tunable), and 1025 nm (fixed)